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Evolution of genome size in streptophyte algae genus Zygnema and its ecological consequences
Lukeš, Jiří ; Čertnerová, Dora (advisor) ; Lučanová, Magdalena (referee)
Genome size is a trait that varies 64,000 × across eukaryotic diversity. However, this variability has nothing to do with the complexity of organisms. The amount of DNA itself is however related to a number of phenotypic traits of organisms. Specifically, studies conducted on plants look for possible connections between genome size and the ecological requirements of individual species. Similar studies in microscopic algae are not abundant and often face a number of methodological problems. This thesis presents genome size data obtained by flow cytometry for 129 representatives of the genus Zygnema belonging to 68 evolutionary lineages. Measurements revealed a genome size range within this genus from 0.22 to 5.06 pg. At the same time, several lines showing considerable variability in genome size were described, which in many cases indicates possible polyploidization in this genus. The identification of an identical GC base pair ratio in selected putative polyploid and monoploid representatives supported the hypothesis of polyploidization. The general correlation of cell size and genome size within this group was also validated. Due to the known phylogenetic relationships between the measured lineages, the genome size evolution in the genus Zygnema was reconstructed. Further, possible relationships...

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